What is the Calibrator for Olink® Target 48 composed of and how is it used in the data analysis?

The Calibrator in Target 48 Cytokine (human) is a pooled human plasma sample with spiked in recombinant antigens for proteins with low endogenous levels to ensure that all 45 proteins are detected within Limit of Quantification. The Calibrator used in Target 48 Mouse Cytokine consists of a buffer with recombinant proteins. In both products the Calibrator is used to adjust the predefined standard curve along the y-axis during development at Olink. The Calibrator is also included in triplicate on each sample plate and used to normalize all samples.

Figure 1: The sample plate layout for Target 48 with the positions for the external controls indicated.