What is the Negative control for Olink® Target 96, Olink® Target 48, Olink® Focus and Olink® Flex composed of and how is it used in the data analysis?

Negative Control is included in triplicate on each plate and consists of buffer run as a normal sample. These are used to monitor any background noise generated when DNA-tags come in close proximity without prior binding to the appropriate protein. The negative controls set the background levels for each protein assay and are used to calculate the limit of detection (LOD).

Figure 1: The sample plate layout for Target 96 with the positions for the external controls indicated.

Figure 2: The sample plate layout for Target 48 with the positions for the external controls indicated.