Novel protein biomarkers for lung adenocarcinoma

Background

A team from Lund University used the  Olink® Target96 Cardiometabolic panel to identify novel protein biomarkers in the exhaled breath particles (EBPs) of patients with lung adenocarcinoma (LUAD). EBPs are naturally shed from the respiratory system during normal breathing and can be collected easily using a particles in exhaled air device (PExA). This study examined whether the protein composition of EBPs could provide a means for non-invasive measurement of potential biomarkers linked to the diagnosis and prognosis of lung cancer.

 

Outcome

EBP samples were collected from LUAD patients before and after surgery aimed at removing the primary tumor and results compared to healthy controls. The data showed that five proteins (CFHR5, MFAP5, PLTP, HGF-R/MET, CA4) were significantly higher in cases vs controls. All of these proteins have established biological links to cancer-related processes. The longitudinal analysis further revealed that PLTP and MET showed a distinct decreasing trend following surgery, losing significance compared to control subjects and possibly indicating tumor removal.

The differences in PLTP and MET at the protein level could also seen at the RNA level in cancer vs control lung tissue samples. Finally, the association of MET with LUAD was also verified in patient plasma using an ELISA (this could not be verified in EBP samples due to the lower sensitivity of the ELISA compared to the Olink assay). While preliminary, these data suggest the possibility of a promising new approach for the diagnosis and post-surgical monitoring of lung cancer.

Andreasson-et-al-2022

Citation

Andreasson J, Bodén E, Fakhro M, et al. Exhaled phospholipid transfer protein and hepatocyte growth factor receptor in lung adenocarcinoma. (2022) Respiratory Research, DOI: 10.1186/s12931-022-02302-4 Respiratory Research, DOI: 10.1186/s12931-022-02302-4

PLTP and MET have been identified as potential biomarkers for LUAD diagnosis and in the evaluation of successful tumor excision

Andreasson et al. (2022)

Peer-reviewed publications citing the use of Olink panels

Olink’s Proximity Extension Assay (PEA) technology has been used for protein biomarker discovery and analysis across a very broad range of disease areas and applications, providing actionable insights into disease biology and helping to drive future development of new and better therapeutics. There are now well over 1000 publications citing the use of our assays and the list is growing rapidly. Please visit our library of publications to see some of the extraordinary work produced by Olink customers.

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