We tested, and confirmed, that Olink® PEA technology is indeed compatible with non-human primate (NHP) plasma. By comparing results from NHP plasma samples to those from human plasma samples run on Olink Explore 3072, we found that that the majority of our protein assays performed just as well in NHP samples as for human plasma samples and passed our rigorous assay quality control measures. These results show that Olink technology presents another means for translational studies and the development of therapeutics and vaccines using NHPs.
NHPs are commonly suggested as the animal model in preclinical and translational studies where developing therapeutics using rodents have failed or in studies aiming to improve vaccines and therapies. Due to the great similarity between the genome and exome of humans and different NHP species, antibodies targeting human proteins should feasibly detect those of NHPs. We therefore sought to examine the detectability and compatibility of NHP plasma proteins with Olink PEA technology.
The inflammation-related study used Olink Explore 3072 to analyze plasma samples from macaques comprising four treatment groups and a control group. The quality metrics of this analysis, together with results on protein detectability, were compared to data generated in another study on human plasma samples analyzed on the same platform.
Olink PEA assays were able to accurately measure the effects of treatment on protein levels in NHP samples and displayed similar quality metrics to that of human plasma
Results showed that NHP plasma samples produced high quality metrics that parallel those obtained in projects with human samples. In addition, protein detection values were similar in both studies, although slightly higher in the human samples. Regardless of these slight differences, Olink PEA assays were able to accurately measure the effects of treatment on protein levels in NHP samples and displayed similar quality metrics to that of human plasma.
Showgy Ma’ayeh, the main author of the application note, emphasizes the importance of these findings for research requiring the use of animal models like NHPs: “Given the importance of NHPs in different fields of research, including transitional medicine, the ability of Olink PEA technology to provide high throughput data on biomarker expression in NHP will be a great tool for understanding human diseases and developing therapeutics and vaccines.”
Concordance and repeatability tests to evaluate lab performance Read the application note to learn more about this study.