Olink assays provide extensive coverage of the plasma proteome and delivers high quality data for approximately 3000 unique proteins. When it comes time to perform data analysis, however, this amount of data can be overwhelming. Olink has therefore developed a web-based application for basic data visualizations and statistical analyses, Olink® Insights Stat Analysis. This application is based around the same tools and methods as are used by the Olink Data Science team and with it you can:
Download results/figures for more focused data analysis
Example PCA plot from the app
To get started, all you will need is an NPX data file delivered to you by Olink Analysis Services, or one generated with NPX Manager or MyData. If you do not have access to an NPX file but would still like to explore the features of the application, you can use a sample data set which is available in the app (go to the About tab and select Use example data).
Please view the video guide to learn more about the features and how to use the statistical analysis app.
You can view or download a pdf of the app user guide.
Before running any experiment, it is vital to design the experiment in a way that makes obtaining relevant results as likely as possible. For this statistical power is key. In this power calculation tool, Olink provides a web-based application to assist in power calculation for a balanced t-test and balanced one-way ANOVA.
Olink® Analyze is an R package that provides a versatile toolbox to enable fast and easy handling of Olink NPX data for your proteomics research. Olink Analyze provides functions for using Olink data, including functions for importing Olink NPX datasets exported from the NPX Manager, as well as quality control (QC) plot functions and functions for various statistical tests. This package is meant to provide a convenient pipeline for your Olink NPX data analysis.
If you have R programming skills, you can access OlinkAnalyze. Download it directly from CRAN, or install the package from R with the following commands: