• CARDIOMETABOLIC

    Gene
    VSIR

    Uniprot
    Q9H7M9

    Protein
    V-type immunoglobulin domain-containing suppressor of T-cell activation

    See alternative names
    Stress-induced secreted protein-1,
    V-set domain-containing immunoregulatory receptor,
    V-set immunoregulatory receptor,
    Platelet receptor Gi24

    Uniprot Function Description

    Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).

    Sample type

    Human EDTA plasma and serum are the recommended sample types. Human citrate plasma and heparin plasma are also accepted. For other samples types e.g cerebrospinal fluid, (CSF), tissue or cell lysates please we recommend Olink Target 96 panels. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

    Precision

    Precision (repeatability) is calculated from linearized NPX values over LOD.

    Within run precision Coefficient of Variation (CV)
    10%
    Between run precision Coefficient of Variation (CV)
    14%

    Analytical measuring range

    The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen. Please note that these figures are for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples.

    LOD (pg/mL)
    1.5
    LLOQ (pg/mL)
    3.1
    ULOQ (pg/mL)
    1563
    Hook (pg/mL)
    6250
    Range (logs)
    2.7

    Dilution factor

    For optimal assay readout, Olink Explore is run using different dilutions of the original samples (undiluted, 1:10, 1:100 or 1:1000). The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

    Dilution factor
    1:10

    Sensitivity plot

    The calibrator curve shown below visualizes the analytical measuring range data based on in vitro measurement of recombinant antigen. Please note that this is shown for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples. The vertical dotted lines represent LLOQ and ULOQ respectively, and the horizontal line indicates the LOD.

    Sample distribution plot

    The plot below shows the levels of protein measured in a number of commercial plasma samples. Healthy subjects are shown in blue and samples obtained from patients with a range of diseases are shown in red. The latter include inflammatory, cardiovascular, autoimmune & neurological diseases, as well as cancer. The data is shown to give a general idea of the sort of data range to expect, but cannot cover all potential levels that may be seen in clinical samples.