Is sample dilution necessary before running the assay?

Each Olink® Target panel is optimized for the expected physiological and pathological ranges found in plasma and serum for the 92 biomarker assays included. A few of our panels are designed to be run diluted (from 1:10 up to 1:2025) so as to fit the physiological ranges within the measurable ranges of our assays, these dilutions are a part of the assay and no pre-dilution is necessary.

These dilutions are set with plasma and serum in mind,  so when running other sample matrices, these dilutions may need adjustment depending on the expected protein concentrations in the specific matrix.

For tissue or cell lysate, we recommend a range from 0.5 – 1.0 µg/µL of total protein concentration.

Our current list of diluted panels is as follows:

  • Cardiometabolic: 1:2025
  • CVD III: 1:100
  • Development: 1:100
  • Metabolism: 1:10

To determine the optimal protein concentration for the specific matrix, it is recommended to run samples in at least two different concentrations. It is important that all samples that are to be analyzed together have the same starting concentration. For more information and guidelines, please contact our support team.

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