What is Olink NPX Signature?
Olink NPX Signature is a data analysis software that is designed for the Olink® Target analysis platform, including Olink Target 48, Olink Target 96, as well as for Olink® Focus panels. It is a standalone Windows software application that lets you import data from the Olink Signature Q100 instrument, process the data (e.g. analyse the data quality, normalize data, and perform statistical analysis) and generate result reports. The data is not required to be annotated or processed before importing it into the Olink NPX Signature software. This software is also compatible with the Biomark™ HD instrument and Olink® NPX Manager software files.
How does it work?
Input data for Olink NPX Signature are run files from Signature Q100, Ct values exported from the Fluidigm Real-Time PCR Analysis software, NPX Manager .oaf project files and NPX Signature .npx study files. After quality control and normalization against the Extension Control, the Inter-Plate Control (IPC) and a correction factor, the output data are obtained in Normalized Protein eXpression (NPX) values. NPX is an arbitrary unit on a log2 scale, where a high NPX value corresponds to high protein concentration.