Expanding access to high-quality NGS-based proteomics
Olink® Reveal now verified for sequencing on MGI T1+ and G400 sequencers
Olink® Reveal continues to expand access to high-quality, NGS-based proteomics. Verification studies have now confirmed that Olink® Reveal data can be sequenced on MGI T1+ and G400 platforms, using optimized protocols that deliver reliable and consistent performance.
Verification overview
The verification compared the performance of MGI T1+ and G400 sequencers with Illumina’s NextSeq 2000 P4 flow cell, assessing consistency in read counts, quantitative correlations, assay variability, and detectability.
Results demonstrated that:
- Read yields on MGI sequencers were comparable to those obtained with the NextSeq 2000 P4 flow cell (Figure 1).
- High correlations indicated consistent quantitative performance between MGI and Illumina sequencing data (Figure 2).
- Coefficient of Variation (CV) differences between platforms were minimal, providing a solid basis to identify meaningful biological patterns (G400 mean CV relative to NextSeq 2000: +0.27%; T1+ mean CV relative to NextSeq 2000: +0.21%).
- Detectability, the proportion of proteins detected above limit of detection (LOD), was comparable across sequencers, indicating proteins are reliably detected regardless of platform.
In addition, evaluation of two-library sequencing on the T1+ confirmed that two Olink® Reveal libraries can be processed simultaneously without significant impact on data quality or precision.
Collectively, these results verify that Olink® Reveal delivers equivalent quantitative performance, reproducibility, and detectability on MGI T1+ and G400 sequencers when compared with Illumina-based workflows.
By extending verified compatibility to MGI T1+ and G400 sequencers, Olink expands access to scalable, NGS-based proteomics—empowering more laboratories to generate actionable insights from 1,000 carefully curated proteins using their existing sequencing infrastructure.
Contact us to learn how to start running Olink® Reveal on your MGI T1+ or G400 sequencer.
Figure 1: Bar chart comparing read yields across MGI T1+, MGI G400, and Illumina NextSeq 2000 P4 flow cells. MGI sequencers show comparable read counts to Illumina, confirming sufficient coverage for Olink® Reveal analysis.
Figure 2: Scatterplots showing Pearson’s correlation between MGI (T1+ and G400) and Illumina NextSeq 2000 sequencing data for Olink® Reveal assays. High global correlation demonstrates quantitative equivalence between platforms, with no systematic assay differences observed.