Emerging Affinity Reagents for High Throughput Proteomics

High throughput proteomic profiling of human plasma is immensely challenging due to the extraordinary complexity and broad dynamic range of plasma protein concentrations. Mass spectrometry is unmatched for its ability to provide unambiguous identification of specific peptides, though its application to plasma proteomics at the population level remains prohibitive due to slow throughput and inability to quantify low abundant proteins without extensive upfront sample separation. ELISA-based approaches in turn have been limited by the unavailability of well-credentialed antibodies for many blood proteins and by issues with cross-reactivity, which significantly hampers the ability to multiplex antibodies for higher throughput analyses.