Serum Protein Profiles Differ with Adalimumab and Ustekinumab Treatment in Moderately to Severely Active Crohn’s Disease
Journal of Crohn's and Colitis, 2025
Venkat S., Zeeman M., Hart A., Bhagat S., Galbraith D., Hoops T., Ufberg P., Izanec J., Freeman T., Mcrae B., Shinzaki S., Jairath V., Panaccione R., Sands B., Branigan P.
| Disease area | Application area | Sample type | Products |
|---|---|---|---|
Immunological & Inflammatory Diseases | Pathophysiology | Serum |  Olink Target 96 |
Abstract
Background and Aims
Adalimumab and ustekinumab are approved for the treatment of moderately to severely active Crohn’s disease (CD); however, comparative data assessing the mechanism of action of tumor necrosis factor (TNFα) and interleukin-12/23p40 blockade in a head-to-head, double-blind CD trial are not available. Here we compared inflammatory serum proteins of biologic-naive patients with moderately to severely active CD treated with adalimumab or ustekinumab who participated in the SEAVUE trial (NCT03464136).
Methods
Serum from CD patients receiving adalimumab (n = 195) or ustekinumab (n = 191), and a separate cohort of healthy controls (n = 40) was evaluated with a targeted inflammation panel, and a high sensitivity IL-22 assay. Differences in temporally regulated proteins were assessed at Weeks 16 and 52 in the context of study endpoints including clinical and endoscopic response.
Results
Expression levels of 79 inflammatory proteins were reliably measured in serum. At Week 0, before receiving study intervention, the overall cohort had similar serum proteomic expression profiles. At Weeks 16 and 52, following treatment with adalimumab or ustekinumab, distinct changes in serum proteins associated with inflammation were observed, including broader suppression of inflammation-related proteins by adalimumab and a reduction of IL-22 observed only with ustekinumab. Reduction of interferon-γ levels by ustekinumab at Week 52 was greater than adalimumab and associated with a decrease in fatigue.
Conclusions
Although patients receiving either adalimumab or ustekinumab reached similar rates of clinical remission at Week 52, the two therapies resulted in unique serum proteomic expression profiles reflecting mechanistic differences. Long-term treatment data is necessary to understand how differences in therapeutic mechanisms are associated with maintenance of remission and changes in patient reported outcomes in the context of inflammatory biomarkers.