Dermal white adipose tissue derived IL-33 regulates interleukin 4/13 expression in myeloid cells during inflammation
Journal of Investigative Dermatology, 2024
Ertel A., Anderegg U., Franz S., Saalbach A.
Disease area | Application area | Sample type | Products |
---|---|---|---|
Immunological & Inflammatory Diseases | Tissue Culture Conditioned Medium | T Target 48 Mouse |
Abstract
Effective tissue response to infection and injury essentially relies on the fine-tuned induction and subsequent resolution of inflammation. Recent research highlighted multiple functions of dermal white adipose tissue (dWAT) beyond its traditional role as an energy reservoir. However, in contrast to other fat depots, there are only limited data about putative immune-regulatory functions of dWAT. Therefore, we investigated the impact of dWAT in the control of an acute skin inflammation. Skin inflammation triggers the activation of dWAT. In turn, soluble mediators of activated dWAT stimulate the expression of numerous genes controlling skin inflammation including the Th2 cell cytokines interleukin-4 (IL-4) and interleukin-13 (IL-13) in myeloid cells in vitro. Consistently, myeloid cells isolated from inflamed skin showed a significant upregulation of IL-4/13 expression compared to those isolated from healthy skin. Mechanistically, we demonstrate that interleukin-33 (IL-33) released from activated dWAT is responsible for IL-4/13 stimulation in myeloid cells. Interestingly, obesity attenuates IL-33 secretion in dWAT during inflammation resulting in decreased IL-4 and IL-13 expression in myeloid cells.
Our data reveal an IL-33 – IL-4/13 signaling cascade initiated from dWAT in a Th2 independent context of inflammation that may contribute to limitation of inflammation. This cascade seems to be disturbed in obese individuals with prolonged inflammation.