Olink

Olink®
Part of Thermo Fisher Scientific

Explore HT

Gene
B4GALT1

Uniprot
P15291

Protein
Beta-1,4-galactosyltransferase 1

See alternative names UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1,
Nal synthase,
N-acetyllactosamine synthase,
Lactose synthase A protein,
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase,
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase,
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Uniprot Function Description

The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Sample type

Recommended sample types are human plasma and serum. For other sample types e.g cerebrospinal fluid, (CSF), tissue or cell lysate, please contact support@olink.com for more information. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

Precision

Precision (repeatability) is calculated from linearized NPX values over LOD.

Within run precision Coefficient of Variation (CV)
11%

Analytical measuring range

The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen.

LOD (NPX)
-4.4
Hooked
No
Hook (NPX)
4.7

Dilution factor

For optimal assay readout, Olink assays are run using different dilutions of the original samples e.g. undiluted, 1:10, or higher. The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

Dilution factor
1:10

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

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