Olink

Olink®
Part of Thermo Fisher Scientific

Why proteomics matters

Why immune biology needs clarity and confident protein insights

Immune responses are coordinated, regulated, and carried out by dynamic protein signaling events. By measuring thousands of cytokines, chemokines, receptors, and other immune-related proteins simultaneously, you gain a clearer view of immune activities, enabling you to pinpoint meaningful immune protein signatures as you study diseases and cohorts.

Immune mechanisms

Understand the cytokines, chemokines, and immune proteins that drive immune response and regulation.

Disease biology

Explore protein signatures associated with infection, autoimmunity, inflammatory diseases, and immune dysregulation.

Immune modulation

Study how therapeutic interventions change immune signaling dynamics and protein signatures.

Biomarker insights

Identify meaningful immune protein signatures that can support translational research and drug development programs.

Biological variation

See how immune protein signatures differ across individuals and disease states.

    Platform comparison
    Scaling your immunology research from discovery to translation

    The scalable Olink proteomics platform helps you move beyond narrow immune marker analysis with the specificity, sensitivity, and scalability needed to study complex immune biology. From broad pathway exploration to focused biomarker follow-up studies, Olink panels enable high-specificity, high-quality, and high-throughput protein insights for immunology research.

    Traditional immune biomarker research

    Olink proteomics advantages

    Focus on a few cytokines or single biomarkers, giving limited view of immune complexity

    Measure thousands of proteins across immune pathways to reveal disease-specific signatures, molecular endotypes, and dynamic pathways

    May struggle to accurately distinguish between closely related immune proteins

    Low cross-reactivity supports highly specific measurement of highly homologous immune proteins

    Have a limited detection range and low sensitivity that can miss low abundance proteins

    Detect proteins, including low expressors, across a dynamic range spanning 10 orders of magnitude

    Require large sample volumes, which is challenging for studies with extremely precious samples

    Requires as little as 1 µL of sample volume

    High variability often requires more replicates for confident interpretation

    Enables high reproducibility

    Research highlights
    Advance your immunology research with Olink proteomics

    Olink proteomics helps you to decode complex immune responses with high-specificity measurements, helping to identify immune protein signatures that impact real biology. Explore how researchers are using Olink solutions to bring greater clarity to their immunology research.

    Delving into the murine immune system with 1 µL of sample

    A conversation on the expanding horizons in using mouse model for autoimmune disease research

    DOWNLOAD

    Studying treatment responses with predictive protein profiles

    A recent study by Prasad et al. used Olink-based plasma proteomics to look for protein profiles that could potentially predict which RA patients would or would not respond to six months of anti-TNF treatment.

    SEE BIOMARKERS

    Immune profiling of inflammatory diseases in the pediatric population

    Research on inflammatory diseases in the pediatric population is often limited by small volume of biospecimens and timely collection of these samples. In this presentation, we discuss the use of Proximity Extension Assay for immune profiling of pediatric inflammatory diseases including Kawasaki disease, multisystem inflammatory syndrome in children, macrophage activation syndrome, and various viral infections.

    WATCH WEBINAR

    Deciphering the immune biology of long COVID

    Long COVID shows persistent immune dysregulation across global cohorts. Proteomic analysis identified 13 shared pathways involving immune stress and altered hematopoiesis, guiding targeted therapies in the LC-REVITALIZE Phase III trial.

    WATCH SESSIONS

    Decoding vaccine responses in human lymph node

    Research focusing on early Innate Immune Response using Olink™ Reveal from Van Anh Vo, PhD.

    WATCH WEBINAR

      Plan and begin your immunology study with ease

      Connect with Olink experts to plan your immunology study, access high-quality proteomics, and move from project setup to scientific insight faster.

      CONTACT US
      Application spotlight
      Explore immune signatures associated with prediction and response

      Comprehensive proteomic profiling can help uncover biomarkers associated with immune responses, disease progression, disease risk and severity, and treatment response. By measuring thousands of proteins across pathways, you can discover molecular endotypes, compare disease-associated signatures, and gain insight into potential stratification and therapy selection strategies for translational research.

      PEA technology
      High specificity, sensitivity and dynamic range

      Olink PEA technology combines the specificity of dual antibodies with the sensitivity of PCR. This unique design enables accurate protein detection without cross-reactivity and delivers a dynamic range of 10 orders of magnitude. With integrated DNA barcodes and NGS readout, researchers can measure over 5,000 proteins across hundreds of samples in a single run.

      Olink users
      Proteomics to decode immune complexity

        Testimonials

        Voices of immunology researchers

        Hear how scientists are leveraging Olink proteomics to unravel immune complexity.

        With its ability to provide precise concentrations of various cytokines in both inflamed and healthy skin, Olink Flex was instrumental in distinguishing between different inflammatory skin conditions. Moreover, while using minimal volumes of skin interstitial fluid, Olink Flex has demonstrated its capability to detect cytokines such as type I Interferons, which are challenging to identify through our next-generation sequencing data.

        Dr. Khashayar Afshari

        Department of Dermatology, UMASS)

        To begin, it’s helpful to have a broad overview of key cytokine types, such as those included in the Olink Target 48 Cytokine panel, which we routinely use in most of our clinical [research] studies. The panel is largely sufficient for the types of cytokines we are interested in, like proinflammatory mediators.

        Dr. Eugenia Ong

        Principal Research Scientist at the Programme in Emerging Infectious Diseases, Duke-NUS Medical School

          Featured reading
          Selected reading for immunology research

          Explore articles and case studies showcasing proteomics advances in immunological research, from large-scale proteomics studies and novel disease insights to translational applications.

          Longitudinal proteomics in HIV controllers

          Longitudinal plasma proteomic signatures of elite and viremic spontaneous HIV controllers

          READ PUBLICATION

          Proteomics in autoimmune diseases

          Improvements in immune/melanocyte biomarkers with JAK3/TEC family kinase inhibitor ritlecitinib in vitiligo

          READ CASE STUDY
          Resources
          Related documents

          Access brochures, technical notes and white papers to support your immunology research.

          DocumentsTypeFile
          Multiple analysis of inflammatory proteins: a comparative study across multiple platforms
          White paper
          Insights from precious samples in inflammatory and infectious disease research
          Flyer
          		Download PDF
          Olink Explore HT - Discover more biology with confidence
          White paper
          Olink Reveal - Proteomics, Unplugged
          Brochure
          Olink Target 48 Immune biomarker profiling with absolute confidence brochure
          Brochure
          Olink Target 48 Mouse Cytokine comprehensive product brochure
          Brochure
          Designing Non-Terminal Longitudinal Mouse Studies
          Ensuring quality with flexibility- Olink® Flex validation & verification
          White paper
          Cytokine changes in the aged tumor microenvironment in a mouse model
          Case study
          		Download PDF
          Access options
          Bringing PEA technology into your research

          Whether you are looking for full-service sample analysis, support from a Certified Service Provider, or want to run the assays in your own lab, you can access Olink solutions in a way that fits your study designs and infrastructure needs.

          Set up in your own lab

          Get expert training on setting up the entire Olink workflow in your lab.

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          Olink analysis services

          Access full-service sample analysis from Olink experts, tailored to your study design and research goals.

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          Find a Service Provider

          Discover the global network of Certified Service providers offering top-tier Olink services.

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