Olink

Olink®
Part of Thermo Fisher Scientific

Explore HT

Gene
IL12A_IL12B

Uniprot
P29459_P29460

Protein
Interleukin-12

See alternative names NK cell stimulatory factor chain 1,
IL-12 subunit p35,
Cytotoxic lymphocyte maturation factor 35 kDa subunit,
IL-12 subunit p40,
Cytotoxic lymphocyte maturation factor 40 kDa subunit,
NK cell stimulatory factor chain 2,
IL12A,
IL12B

Uniprot Function Description

Interleukin-12 is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). Interleukin-12 can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Sample type

Recommended sample types are human plasma and serum. For other sample types e.g cerebrospinal fluid, (CSF), tissue or cell lysate, please contact support@olink.com for more information. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

Precision

Precision (repeatability) is calculated from linearized NPX values over LOD.

Within run precision Coefficient of Variation (CV)
9%

Analytical measuring range

The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen.

LOD (NPX)
-2.8
Hooked
No
Hook (NPX)
5.9

Dilution factor

For optimal assay readout, Olink assays are run using different dilutions of the original samples e.g. undiluted, 1:10, or higher. The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

Dilution factor
1:1

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

Search all Biomarkers