Olink

Olink®
Part of Thermo Fisher Scientific

Explore HT

Gene
HSD17B2

Uniprot
P37059

Protein
17-beta-hydroxysteroid dehydrogenase type 2

See alternative names 20 alpha-hydroxysteroid dehydrogenase,
E2DH,
Estradiol 17-beta-dehydrogenase 2,
Microsomal 17-beta-hydroxysteroid dehydrogenase,
Short chain dehydrogenase/reductase family 9C member 2,
Testosterone 17-beta-dehydrogenase

Uniprot Function Description

Catalyzes the NAD-dependent oxidation of the highly active 17beta-hydroxysteroids, such as estradiol (E2), testosterone (T), and dihydrotestosterone (DHT), to their less active forms and thus regulates the biological potency of these steroids. Oxidizes estradiol to estrone, testosterone to androstenedione, and dihydrotestosterone to 5alpha-androstan-3,17-dione. Also has 20-alpha-HSD activity.

Sample type

Recommended sample types are human plasma and serum. For other sample types e.g cerebrospinal fluid, (CSF), tissue or cell lysate, please contact support@olink.com for more information. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

Precision

Precision (repeatability) is calculated from linearized NPX values over LOD.

Within run precision Coefficient of Variation (CV)
N/A

Analytical measuring range

The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen.

LOD (NPX)
3.1
Hooked
No
Hook (NPX)
7.1

Dilution factor

For optimal assay readout, Olink assays are run using different dilutions of the original samples e.g. undiluted, 1:10, or higher. The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

Dilution factor
1:1

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

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