Olink

Olink®
Part of Thermo Fisher Scientific

Explore HT

Gene
NCF1C_NCF1

Uniprot
A8MVU1_P14598

Protein
Neutrophil cytosol factor 1/1C

See alternative names 47 kDa autosomal chronic granulomatous disease protein,
47 kDa neutrophil oxidase factor,
NCF-47K,
Neutrophil NADPH oxidase factor 1,
Nox organizer 2,
Nox-organizing protein 2,
SH3 and PX domain-containing protein 1A,
p47-phox

Uniprot Function Description

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).NCF1C May be required for activation of the latent NADPH oxidase (necessary for superoxide production).

Sample type

Recommended sample types are human plasma and serum. For other sample types e.g cerebrospinal fluid, (CSF), tissue or cell lysate, please contact support@olink.com for more information. Please note that protein expression levels are expected to vary in different sample types and certain assays may be affected by interfering substances such as hemolysate.

Precision

Precision (repeatability) is calculated from linearized NPX values over LOD.

Within run precision Coefficient of Variation (CV)
13%

Analytical measuring range

The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen.

LOD (NPX)
-2.7
Hooked
No
Hook (NPX)
8.8

Dilution factor

For optimal assay readout, Olink assays are run using different dilutions of the original samples e.g. undiluted, 1:10, or higher. The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.

Dilution factor
1:1

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

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