Olink

Olink®
Part of Thermo Fisher Scientific

Target 48 Cytokine

Gene
CCL3

Uniprot
P10147

Protein
C-C motif chemokine 3

See alternative names LD78-alpha(4-69),
Tonsillar lymphocyte LD78 alpha protein,
Small-inducible cytokine A3,
SIS-beta,
PAT 464.1,
Macrophage inflammatory protein 1-alpha,
G0/G1 switch regulatory protein 19-1

Uniprot Function Description

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Sample type

Recommended sample types are plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsies, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary between different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

Precision

Precision (repeatability) is calculated from quantified data (pg/mL) within the limits of quantification: LOQ.

Within run precision Coefficient of Variation (CV)
4%
Between run precision Coefficient of Variation (CV)
4%

Analytical measuring range

The technical data reported below refers to the measured values generated during the assay validation process using recombinant antigens. Per default, Olink Target 48 products deliver data in absolute protein concentrations (pg/mL) using the Calibrator built into the system.

LOD (pg/mL)
0.030
LLOQ (pg/mL)
0.030
ULOQ (pg/mL)
1953
Hook (pg/mL)
3906
Range (logs)
4.8

Sensitivity plot

The calibrator curve shown below visualizes the analytical measuring range determined during the validation process using recombinant antigen. Please note that this is shown for reference only and CANNOT be used to e.g. convert NPX values to absolute concentrations. The vertical dotted lines represent lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) respectively. The solid horizontal line indicates the limit of detection (LOD).

SensitivityPlot

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

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