Target 48 Cytokine
Gene
CCL4
Uniprot
P13236
Protein
C-C motif chemokine 4
See alternative names HC21,
G-26 T-lymphocyte-secreted protein,
Lymphocyte activation gene 1 protein,
MIP-1-beta(1-69),
Macrophage inflammatory protein 1-beta,
PAT 744,
Protein H400,
SIS-gamma,
Small-inducible cytokine A4,
T-cell activation protein 2
Uniprot Function Description
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Sample type
Recommended sample types are plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsies, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary between different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.
Precision
Precision (repeatability) is calculated from quantified data (pg/mL) within the limits of quantification: LOQ.
Analytical measuring range
The technical data reported below refers to the measured values generated during the assay validation process using recombinant antigens. Per default, Olink Target 48 products deliver data in absolute protein concentrations (pg/mL) using the Calibrator built into the system.
Sensitivity plot
The calibrator curve shown below visualizes the analytical measuring range determined during the validation process using recombinant antigen. Please note that this is shown for reference only and CANNOT be used to e.g. convert NPX values to absolute concentrations. The vertical dotted lines represent lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) respectively. The solid horizontal line indicates the limit of detection (LOD).
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center