Target 48 Mouse Cytokine
Gene
Cx3cl1
Uniprot
O35188
Protein
Fractalkine
See alternative names ABCD-3,
C-X3-C motif chemokine 1,
CX3C membrane-anchored chemokine,
Neurotactin,
Small-inducible cytokine D1
Uniprot Function Description
Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:10187784, PubMed:18971423). The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:10187784, PubMed:10382755, PubMed:18971423, PubMed:9177350). Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:10382755, PubMed:9177350). Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (By similarity). In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (By similarity). In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (By similarity).
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.
The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.
Sample type
Recommended sample types are plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsies, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary between different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.
Precision
Precision (repeatability) is calculated from quantified data (pg/mL) within the limits of quantification: LOQ.
Analytical measuring range
The technical data reported below refers to the measured values generated during the assay validation process using recombinant antigens. Per default, Olink Target 48 products deliver data in absolute protein concentrations (pg/mL) using the Calibrator built into the system.
Sensitivity plot
The calibrator curve shown below visualizes the analytical measuring range determined during the validation process using recombinant antigen. Please note that this is shown for reference only and CANNOT be used to e.g. convert NPX values to absolute concentrations. The vertical dotted lines represent lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) respectively. The solid horizontal line indicates the limit of detection (LOD).
Sample distribution plot
The plot below shows the levels of protein measured in a number of mouse samples (for further information please see the Validation Data Document which can be found in the Document download center). The data is shown to give an idea of the data range to expect but cannot cover protein levels that may be seen under all potential conditions.
Plasma
Mouse EDTA plasma samples were acquired for assay performance evaluation. Protein values indicate range, and group comparisons should be interpreted with caution.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center