Olink

Olink®
Part of Thermo Fisher Scientific

Target 48 Mouse Cytokine

Gene
Il1b

Uniprot
P10749

Protein
Interleukin-1 beta

Uniprot Function Description

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

Sample type

Recommended sample types are plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsies, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary between different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

Precision

Precision (repeatability) is calculated from quantified data (pg/mL) within the limits of quantification: LOQ.

Within run precision Coefficient of Variation (CV)
4%
Between run precision Coefficient of Variation (CV)
3%

Analytical measuring range

The technical data reported below refers to the measured values generated during the assay validation process using recombinant antigens. Per default, Olink Target 48 products deliver data in absolute protein concentrations (pg/mL) using the Calibrator built into the system.

LOD (pg/mL)
0.320
LLOQ (pg/mL)
0.320
ULOQ (pg/mL)
9679
Hook (pg/mL)
20000
Range (logs)
4.4

Sensitivity plot

The calibrator curve shown below visualizes the analytical measuring range determined during the validation process using recombinant antigen. Please note that this is shown for reference only and CANNOT be used to e.g. convert NPX values to absolute concentrations. The vertical dotted lines represent lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) respectively. The solid horizontal line indicates the limit of detection (LOD).

SensitivityPlot

Sample distribution plot

The plot below shows the levels of protein measured in a number of plasma samples (for further information please see the Validation Data Document which can be found in the Document download center). The data is shown to give an idea of the data range to expect but cannot cover protein levels that may be seen under all potential conditions.

SampleDistributionPlot

Biomarker Validation Data

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