Olink

Olink®
Part of Thermo Fisher Scientific

Target 48 Neurodegeneration

Gene
RTN4R

Uniprot
Q9BZR6

Protein
Reticulon-4 receptor

See alternative names Nogo-66 receptor,
Nogo receptor

Uniprot Function Description

Receptor for RTN4, OMG and MAG (PubMed:12037567, PubMed:12068310, PubMed:12426574, PubMed:12089450, PubMed:16712417, PubMed:18411262, PubMed:12839991, PubMed:19052207). Functions as receptor for the sialylated gangliosides GT1b and GM1 (PubMed:18411262). Besides, functions as receptor for chondroitin sulfate proteoglycans (By similarity). Can also bind heparin (By similarity). Intracellular signaling cascades are triggered via the coreceptor NGFR (PubMed:12426574). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:16712417, PubMed:22325200). Mediates axonal growth inhibition (PubMed:12839991, PubMed:19052207, PubMed:28892071). Plays a role in regulating axon regeneration and neuronal plasticity in the adult central nervous system. Plays a role in postnatal brain development. Required for normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated via interaction with MAG. Acts in conjunction with RTN4 and LINGO1 in regulating neuronal precursor cell motility during cortical development. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200).

Sample type

Recommended sample types are plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsies, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary between different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

Precision

Precision (repeatability) is calculated from quantified data (pg/mL) within the limits of quantification: LOQ.

Within run precision Coefficient of Variation (CV)
5%
Between run precision Coefficient of Variation (CV)
7%

Analytical measuring range

The technical data reported below refers to the measured values generated during the assay validation process using recombinant antigens. Per default, Olink Target 48 products deliver data in absolute protein concentrations (pg/mL) using the Calibrator built into the system.

LOD (pg/mL)
44.9
LLOQ (pg/mL)
44.9
ULOQ (pg/mL)
11084
Hook (pg/mL)
64000
Range (logs)
2.4

Sensitivity plot

The calibrator curve shown below visualizes the analytical measuring range determined during the validation process using recombinant antigen. Please note that this is shown for reference only and CANNOT be used to e.g. convert NPX values to absolute concentrations. The vertical dotted lines represent lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ) respectively. The solid horizontal line indicates the limit of detection (LOD).

SensitivityPlot

Sample distribution plot

The plot below shows the levels of protein measured in a number of human samples (for further information please see the Validation Data Document which can be found in the Document download center). The data is shown to give an idea of the data range to expect but cannot cover protein levels that may be seen under all potential conditions.

Plasma

Human EDTA plasma samples were acquired for assay performance evaluation. Protein values indicate range, and group comparisons should be interpreted with caution.

SampleDistributionPlasmaPlot

Cerebrospinal fluid (CSF)

Human CSF samples were acquired for assay performance evaluation. Protein values indicate range, and group comparisons should be interpreted with caution.

SampleDistributionCSFPlot

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

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