Olink Target 96
Olink Target 48
Olink Focus
Olink Flex
NPX Signature
Quality control is done using Olink NPX Signature software. It ensures reliable data by using three internal controls in all samples and external controls in each run.
Line-in-chip detection checks for assays with missing signal in the qPCR. If there are more than five in a sample, the sample is considered failed.
Sample Evaluation: Ensures that internal controls are measured consistently across all samples. Incubation Control and Detection Control should not deviate more than ±0.3 NPX from the plate median. Samples outside of specification get a warning flag in Sample QC.
Assay Evaluation: Based on external controls. All products require:
- minimum two valid Negative Controls
- minimum two valid Calibrators / Interplate Controls (IPC)
- CV less than 30% for Calibrators / IPC
In addition, Target 48, Flex and Focus require minimum two valid Sample Controls
- CV less than 30% for the Sample Controls
- Sample Control accuracy median less than 30% from expected concentration values
Assay is failed if the minimum number of valid Calibrators / IPC replicates is not reached. For all other assay QC requirements, the assay gets a warning flag in Assay QC.
Run Evaluation: All products require:
- minimum two valid Negative Controls
- minimum two valid Calibrators / IPC
- number of flagged or failed assays are less than 1/9 of assays
- number of flagged or failed samples are less than 1/6 of customer samples
- variation in internal controls must have median absolute deviation less than
- 0.23 for customer samples
- 0.25 for external controls
In addition, Target 48, Flex and Focus require:
- minimum two valid Sample Controls
All run QC requirements generate a warning flag in the Run QC. RunQC flags are only shown in UI and not in the exported data.